Biopolymers and cell. 2008; 24 (6): 508 - 512

Efficient gene delivery into mammalian cells by baculovirus vector in vitro

I. N. Vagyna, O. V. Anopriyenko, O. A. Zaharuk, V. F. Gorchev, L. I. Strokovska, A. P. Solomko

The recombinant baculovirus vector with EGFP reporter gene under the control of strong CAG promoter cassette was used for transduction of normal (HEK293) and tumor (HeLa) human cell lines. Dependence of transduction efficiency on the virus dose, time of virus incubation, temperature and cell type is shown. Transient reporter gene expression gradually diminished and was detected in single cells on the 15th day after transduction.

Keywords: recombinant baculovirus, AcMNPV, transduction, transient expression, gene therapy.