Biopolymers and cell. 2008; 24 (6): 503 - 507

Bioinformatic search for potential phosphorylation sites of melusin – integrin β1-binding protein

I. V. Kroupskaya, L. M. Kapustian, L. L. Sidorik

Phosphorylation is one of the most frequently occurring posttranslational modifications in proteins. It plays an essential role in transferring outside signals into a cell and regulates different cellular processes such as growth, metabolism, proliferation, motility and differentiation. Melusin is a stress response protein which strictly reacts to the threshold levels of mechanic stress and activates cardiomyocytes signaling pathways. The search for potential sites of melusin phosphorylation was performed using bioinformatic analysis of primary protein sequences. The comparative bioinformatic analysis of possible phosphorylation sites, evolutionary and structural motifs has identified Ser₃₂₆,Ser₃₂₉ and Ser₃₃₄ as the most likely sites for phosphorylation of melusin by protein kinase CK2 in cardiamyocytes.

Keywords: melusin, C-domain, secondary structure, 3D-structure, cell signaling pathways, prediction phospthorylation sites.