Biopolymers and cell. 2008; 24 (6): 453 - 462

Noncanonical complexes of mammalian eEF1À with various deacylated tRNAs

P. V. Futernyk, B. S. Negrutskii, G. V. El’ska

The formation of noncanonical complexes of two eEF1A isoforms with different types of deacylated tRNAs was examined and apparent dissociation constants were measured. A higher affinity to tRNA was found for the tissue specific isoform of elongation factor eEF1A2, as compared with that of eEF1A1. For the first time the formation of noncanonic complex of eEF1A with initiator tRNAiMet was found to be possible. A specific role of some tRNA sites in the interaction with eEF1A was discovered.

Keywords: translation, elongation factor 1A, channeling.