Biopolymers and cell. 2008; 24 (4): 323 - 332

Identification of chromosome 3 epigenetic and genetic abnormalities and gene expression changes in ovarian cancer

V. V. Gordiyuk, G. V. Gerashchenko, I. Ya. Skrypkina, O. V. Symonchuk, T. V. Pavlova, D. D. Ugryn, E. P. Manzhura, G. O. Vakulenko, E. R. Zabarovsky, A. V. Rynditch, V. I. Kashuba

DNA-microarray technology comprising NotI-linking clones has been used to check ovarian cancer cells for genetic and epigenetic changes. Analysis of samples from 22 patients revealed methylations, deletions and amplifications in 92 out of 181 NotI clones. For 32 gene loci these changes have been shown in more than 30 % of tumor samples that specifies a high probability of these genes involvement in the ovarian cancer development. For two genes, GORASP1 and GNAI2, the decrease in their expression has been confirmed by Northern blot analysis. Aberrations of 16 genes and loci unknown previously to be involved in the development of ovarian cancer have been detected.

Keywords: NotI-microarrays, human chromosome 3, DNA methylation, gene expression, ovarian cancer.