Biopolymers and cell. 2008; 24 (4): 294 - 299

Receptors for fibrin fragments on neuroendocrine PC-12 cells

N. Y. Chorna, Y. I. Petrova, O. M. Kalashnik, T. M. Platonova, S. Cederholm-Williams , M. V. Skok

By means of flow cytometry and cell immunoenzyme assay the neuron-like cells PC-12 are shown to express α_νβ₃ integrin responsible for the binding of these cells with fibrinogen and D, DD, E fibrin fragments. Each cell contains about 1×10⁶ D fragment binding sites with the affinity constant of 8.12×10⁶ M^–1 , the binding is blocked by the RGD-containing peptide. The receptors are internalized at physiological temperature renewing on the plasma membrane later. Preincubation with the fibrin fragments results in the increase in receptors number on the cell surface. The D, DD and E fibrin fragments adsorbed on the plastic surface favor PC-12 cell proliferation that may explain a positive effect of fibrin on nerve regeneration.

Keywords: PC-12 cell line, D, DD and E fibri(oge)n) fragments, integrins.