Biopolymers and cell. 2008; 24 (3): 260 - 265

6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase mRNA expression in streptozotocin-diabetic rats

V. G. Mykhalchenko, K. Tsuchihara, D. O. Minchenko, H. Esumi, O. M. Prystupiuk, O. H. Minchenko

We have determined that changes in the expression of 6-Phosphofructo-2-Kinase/Fructose-2,6-Bisphosphatase mRNA in streptozotocin-diabetic rats vary in different organs. The expression of different splice variants of PFKFB-3 mRNA is organ specific and changes in diabetes. We have identified new splice variant of PFKFB-3 mRNA which has catalytic domains identical to those of the main isoform and other alternative splice variants of PFKFB-3 but differs by the length of C-terminus. Results of this investigation support possible role of PFKFB-3 isozymes in adaptation of the cells to disordered carbohydrate metabolism resulting from the insulin deficiency.

Keywords: PFKFB-3 mRNA; alternative splicing, lung, brain, heart, diabetes, rats