Biopolymers and cell. 2008; 24 (3): 253 - 259

Phosphatidylcholine and phosphatidylinositol are allosteric regulators of 5-lipoxygenase from potato tubers

G. I. Kharitonenko, O. V. Kharchenko

Kinetic constants of the linoleic acid oxidation catalized by purified 5-lipoxygenase from Solanum tuberosum tubers have been determined. Hydrophilic and hydrophobic forms of the enzyme have been shown to bind linoleic acid with positive cooperativity in micellar system. Phosphatidylcholine and phosphatidylinositol binding to lipoxygenase results in the release of the substrate from the enzyme, causing the decrease or increase of the reaction rate, respectively. Presented findings imply the allosteric regulation of the 5-lipoxygenase activity by these phospholipids.

Keywords: 5-lipoxygenase, phospholipids, allosteric regulation.