Biopolymers and cell. 2008; 24 (2): 171 - 175

Synthesis of fluorescently labeled oligonucleotide conjugate with transport peptide on modified Silochrom-2 support

I. Ya. Dubey

Synthesis of triple conjugate of oligonucleotide with transport peptide and fluorophore is described. 5'-aminoalkyl-modified 3'-fluorescein-labeled 18-mer oligonucleotide, complementary to the region of E. coli b-galactosidase gene was prepared by solid phase synthesis on fluorescein-modified support Silochrom-2. Its treatment with iodoacetic anhydride led to the formation of iodoacetamide derivative. The latter reacted specifically with cystein-containing 10-mer peptide, a fragment of the transport domain of large T-antigen of SV40 virus, to provide high yield of target hybrid.

Keywords: oligonucleotide conjugates, transport peptides, fluorescein.