Biopolymers and cell. 2008; 24 (2): 129 - 134

PCR-marking of genes of Cbf-family, controlling low temperature acclimatization of barley and soft wheat

I. A. Balashova, M. S. Balvinska, V I. Fait, M. V. Galaeva, Yu. M. Sivolap

Polymorphism in the genotypes of barley and soft wheat, contrast in high and low temperature tolerance, was detected by STS-PCR-analysis with primers, directed to Cbf2 gene. It is supposed that polymorphic DNA-loci are associated with the presence of Cbf2 gene in different allelic state in genotypes with high and low cold stress tolerance.

Keywords: barley, wheat, frost resistance, Cbf-genes, STS-PCR.