Biopolymers and cell. 2007; 23 (6): 501 - 510

Optimization of sucrose measurement working procedure in real samples using conductometric enzyme biosensor

V. N. Peshkova, A. A. Soldatkin, S. V. Dzyadevych

The working procedure of sucrose and glucose conductometric biosensors with real samples of juices and sweet drinks has been presented. For the purpose of sensitivity and stability improvement of the sucrose biosensor, optimal enzyme proportions and concentrations in its bioselective element have been chosen. Different variants of determination of sucrose and glucose in real samples using the biosensor have been considered. The sucrose and glucose measurement has been carried out in juices and sweet drinks. The method suggested could be used in food industry for the production control and optimization.

Keywords: conductometric enzyme biosensor, immobilized enzymes, sucrose, glucose.