Biopolymers and cell. 2007; 23 (5): 405 - 409

Protein-lipid and protein-protein interactions of Bcr PH domain

D.O. Miroshnychenko, A.M. Dubrovska, G.D. Telegeev, S.S. Maliuta

Bcr is a partner of Abl in reciprocal translocation t(9;22) which leads to Philadelphia chromosome formation. In the present study we analyzed Bcr PH domain functions and determined lipid specificity of PH domain in vitro. The technical approach for determining PH domain protein-protein interactions using mass-spectrometry was developed. Interactions with SMC1, β-tubulin, zizimin1 and PLCe were confirmed in vitro and in vivo.

Keywords: Bcr-Abl, PH domain, mass spectrometry.