Biopolymers and cell. 2007; 23 (5): 379 - 385

Far Western blot as a tool for identification of interaction between ribosomal proteins and translation elongation factor 1A

T. V. Budkevich, B. S. Negrutskii

We have used Far Western blot technique to determine several proteins from 60S ribosomal subunit from rabbit liver which are capable of interacting with homologous translation elongation factor 1A. They belong to phosphoprotein P0.P1/P2 complex situated near A site region of 80S ribosome. It was also shown that eEF1A interacts with several proteins of small subunit of 80S ribosomes. It is important since none of 30S subunit proteins binds elongation factor EF-Tu. Identification of the above-mentioned proteins is the aim of future investigations.

Keywords: ribosomal proteins, elongation factor 1A, eukaryotic translation.