Biopolymers and cell. 2007; 23 (4): 338 - 346

Genetic instability of mouse embryonic germinative G1 cell line and disfunction of the mitotic checkpoint and p53

A. P. Iatsyshyna, S. M. Kvasha, O. V. Pidpala, T. P. Ruban, I. M. Vagina, L. L.Lukash

The ability of cells of mouse G1 cell line to survive after treatment with different concentrations of colchicine has been analysed. We have shown that G1 cells at 26^th and 114^th passages are less sensitive to the treatment with colchicine than BALB/c mouse embryonic fibroblasts. These results indicate attenuation of the mitotic checkpoint of G1 cells. The expression of p53 has been detected at low levels in cells of G1 cell line and its sublines G1-OA and G1-T by Western blotting. It has been shown by immunoprecipitation that G1 cells contain wild type and mutant p53 that could lead to the disfunction of p53. Genetic instability of G1 cell line is possibly associated with the failure of the mitotic checkpoint and functional inactivation of p53.

Key words: mouse cell l in vitro, mitotic checkpoint, chromosomal instability, p53.