Biopolymers and cell. 2007; 23 (3): 250 - 254

The expression of O⁶-methylguanine-DNA methyltransferase in the spontaneously immortalized mouse cell line G1 and its sublines G1-OA and G1-T

A. P. Iatsyshyna, V. V. Lylo, O. V. Pidpala, T. P. Ruban, I. M. Vagina, L. L. Lukash

The expression of Mgmt gene was investigated by Western blot analysis in the spontaneously immortalized mouse cell line G1 and in its sublines G1-OA and G1-T at different passages of in vitro cultivation. The highest level of Mgmt expression has been revealed in G1-T subline cells and the lowest – in G1-OA subline cells. The increase in the level of DNA repair enzyme Mgmt was observed in the cells of mouse cell line G1 as well as in its subline G1-OA at later passages of in vitro cultivation. Since the G1-OA subline is characterized by the highest frequencies of chromosomal aberrations, micronucleate and multinucleate cells, it is possible to suppose a role of deficiency of DNA repair enzyme Mgmt in the increase of the chromosomal aberration level in G1-OA subline.

Keywords: mammalian cells in vitro, O⁶-methylguanine-DNA methyltransferase (Mgmt), chromosomal aberrations