Biopolymers and cell. 2007; 23 (2): 143 - 148

Modification of immune SPR biosensor surface at nonylphenol analysis

A. V. Demchenko, M. F. Starodub

The influence of the state of transducer surface of immune SPR biosensor on the sensitivity and stability of its "direct" detection of nonylphenol in solutions has been investigated. The biosensor surface was modified with polyelectrolytes, dodecanethiol, and dextran sulfate and glutaraldehyde. It was shown that the highest sensitivity (up to 10 ng/ml) and stability of the biosensor response were achieved at the surface modification with dodecanthiol and simultaneous creation of intermediate layer with protein A Staphylococcus aureus for the orientation of active sites of specific antibodies toward solution.

Key words: nonylphenol, immune biosensor, polyelectrolytes, dodecanethiol, dextran sulfate, glutaraldehyde.