Biopolymers and cell. 2007; 23 (2): 93 - 99

The development of DNA-vaccine against classical swine fever

I. A. Pokholenko, T. O. Ruban, O. M. Sukhorada, O. M. Deriabin, T. G. Tytok, V. A. Kordium

The development of a DNA-vaccine against classical swine fever (CSF) is a perspective direction, because it gives an opportunity to develop a marker vaccine due to use of a part of protective antigen molecule, and to induce effectively both cellular and humoral immune response. In this study a recombinant plasmid, containing the fragment of E2 gene of CFS virus (CSFV) in eukaryotic expression vector, has been developed. It has been demonstrated that the fragment of E2 protein of CSFV is expressed in CHO-K1 cells from the developed recombinant plasmid pTR-BKneo^-, and we suggest that the protein possesses the post-translational modifications. The data obtained are in favor of the created model DNA-vaccine able to induce humoral immune response to fragment of E2 protein of CSFV.

Key words: CSFV, DNA-vaccine, E2 glycoprotein of CSFV, immunization, humoral immune response.