Biopolymers and cell. 2007; 23 (2): 86 - 92

Stability of the genome of highly productive Rauwolfia serpentina Benth. K-27 cell line at changing maintenance conditions

K. V. Spiridonova, D. M. Adnof, I. O. Andreev, V. A. Kunakh

Genetic analysis of the tissues of long-term passaged strain K-27 of R. serpentina, maintained as surface or submerged cultures on the media of different composition for 4 passages, has been carried out via RAPD-PCR. Changes in the maintenance conditions were found to result in minor rearrangements of strain K-27 genome at the molecular level. However, the switch from the urface maintenance to the submerged one appears to affect the genome of cultured tissues more significantly as compared with the changes in the nutrient medium composition. Introduction of the additional subculture on the agarified medium of the intermediary composition preceding to the switch to submerged culture seems to allow reducing the level of genome changes.

Keywords: plant tissue culture, Rauwolfia serpentina, plant genome, RAPD-analysis.