Biopolymers and cell. 2006; 22 (6): 439 - 445

The distribution of injected plasmid DNA throughout the organs and the expression of human apoA-1 gene in vivo

Yu.M. Gilchuk, D.M. Irodov, P.L. Starokadomskyy, I.M. Pishel, O.K. Toporova, S.M. Novikova, V.A. Kordium

The elaboration of methods of gene delivery into somatic cells is the foundation of gene therapy development. There is necessity to develop new and more effective vectors as well as corresponding methods of gene delivery into the target tissues. The analysis of distribution of vector molecules in tissues and transgene expression are the constituent parts of evaluation of effectiveness of gene therapy methods for a target gene delivery. The study on the transfer of the plasmid DNA, containing human apoA-1 gene, in complex with polyetilenimine (PEI) into rat and rabbit liver parenchyma has been performed. The expression of human apoA-1 gene in vivo has been examined.

Key words: apolipoprotein A-1, polyetilenimine, transgene expression, transgene distribution.