Biopolymers and cell. 2006; 22 (3): 236 - 242

Stabilization of glucose oxidase by low temperature in solution and as a component of biological sensor

V. I . Grischenko, O. A. Nardid, K. D. Rozanova, M. I. Schetinsky, E .I. Naumenko, S. V. Dzyadevych

The effect of freeze-thawing with various cooling rates on structural state of glucose oxidase using low temperatures has been studied for searching the ways of long-term preservation of biosensors. Such cryoprotectors as glycerol, 1,2-propane diol and DMSO were used for complete preservation of the enzyme properties in the process of low temperature preservation. The glycerol and 1,2-propane diol proved to be the most suitable for low temperature preservation of the glucose oxidase sensor that enabled to protect not only the protein part of biosensor but the biosensor protective surface as well.

Keywords: glucose oxidase, biosensor, low temperatures, cryoprotectants.