Biopolymers and cell. 2006; 22 (3): 210 - 216

Study of transient expression of human apoA-1 gene under control of various promoters in CHO-K1 cells

Y. M. Gilchuk, O. M. Sukhorada, T. A. Ruban, O. K. Toporova, V. A. Kordyum

The recombinant plasmids containing the genomic human apoA-1 gene under the transcriptional control of hybrid promoters, namely, the cytomegalovirus immediate early enhancer/chicken β-actin promoter and first intron (CAG promoter) and cytomegalovirus immediate early enhancer/promoter with intron A (hCMVintronA promoter) have been constructed. The transient expression of human apoA-1 gene has been studied in CHO-K1 cells using designed and pTRapo vectors. The expression of the transgene for transfected with pTRapo and pTRapochCMVintronA CHO-K1 cells have been shown.

Keywords: transient expression, apolipoprotein A-1, CHO-K1.