Biopolymers and cell. 2006; 22 (2): 136 - 142

Alterations in a soluble protein pattern and a quantity of stress proteins HSP90 and HSP70 in pea seedlings in response to clinorotation

L. Ye. Kozeko

The influence of clinorotation (a method of partial simulation of the biological effects of microgravity under the earth conditions) on protein spectrum of pea seedlings (Pisum sativum L.) has been investigated. The germination of seeds under horizontal clinorotation (2 rpm) for 3 days resulted in disorientation and some slowing down of seedling growth in comparison to the stationary vertical control. It has been shown that the protein content, including heat-shock proteins HSP90 and HSP70, abundant in dry seeds and the level of which is decreased during germination, remained higher in rotated seedlings comparatively to the control ones. The supposition about deceleration of their storage protein hydrolysis at clinorotation has been made. The complex character of the effect of horizontal clinorotation has been shown by comparing to vertical clinorotation effect on the HSP90 and HSP70 quantities, which testifies to plant reaction to both rotation sensu stricto and altered gravity. The obtained data confirm our assumption about HSP90 and HSP70 participation in the plant adaptation to clinorotation.

Key words: clinorotation, Pisum sativum L., seed germination, protein pattern, heat-shock proteins, stress.