Biopolymers and cell. 2005; 21 (5): 446 - 453

Analysis of unstructured regions of human cytoplasmic tyrosyl-tRNA synthetase by methods of bioinformatics

K. A. Odynets, A. I. Kornelyuk

The prediction of unstructured regions of mammalian cytoplasmic tyrosyl-tRNA synthetase is carried out by bioinformatics methods using 15 web-servers. High probability of unfolded state for flexible “KMSKS» loop of catalytic centre (residue Pro216-Lys231), which getting certain conformation during catalytic act is shown. For the region of intermodule linker (residues Asp343-Glu359) the highest probability of its unstructured state is shown. The comparison of these data with B-factor values for С_a-atoms of crystallographic structures of N- and C-terminal modules shows a strong correlation between the bioinformatics and X-ray analyses data. The presence of flexible intermodular linker is a characteristic feature of proteins which contain the EMAP II-like-terminal module. The hypothesis is proposed about a possible conformational rearrangement of this linker region which may be essential upon the complex formation between these proteins and tRNAs.