Biopolymers and cell. 2005; 21 (4): 365 - 369

Preparation of bifunctional silica polymer support for the synthesis of 3'-labeled oligonucleotides

L. V. Dubey, I. Ya. Dubey

Preparation of a silica polymer support with a linker arm containing protected amino and hydroxy groups is described. O-Benzotriazol-1-yl-N,N,N'‚N'-tetramethyluronium hexafluorophosphate (HBTU) in the presence of 1-hydroxybenzotriazole (HOBT) was used as an efficient activating reagent at several steps of the polymer synthesis. This bifunctional support is suitable for the preparation of 3'-amino-modified oligonucleotides for post-synthetic conjugation, as well as for the direct solid phase synthesis of 3'-labeled oligonucleotides. The fluorescein-modified silica polymer was synthesized.

Key words:  polymer supports, onium reagents, labeled oligonucleotides.