Biopolymers and cell. 2005; 21 (3): 275 - 285

Potentiometric biosensor for detection of potato glycoalcaloids: control of its analytical characteristics, comparison with thin-layer chromatography

A. A. Nazarenko, A. P. Soldatkin, O. F. Sosovskaya, I. V. Benilova, Ya. I. Korpan

Butyrylcholinesterase (BuChE) of different origin along with variations of the time of the enzyme immobilization on the potentiometric transducer surface are offered to control analytical characteristics of the biosensor, based on pH-sensitive field effect transistor with immobilized BuChE, for detection of the potato glycoalcaloids. Utilization of ethylendiaminetetracetate (a complexon of heavy metal ions) and phosphotriesterase (an enzyme capable of exclusive disintegration of phosphororganic pesticides) is shown to enable the selective determination of glycoalcaloids at the background of heavy metal ions and phosphororganic pesticides. 14 potato varieties cultivated in Ukraine have been tested by the biosensor and thin-layer chromatography the correlation between both methods being 0.74.

Key words: biosensor, glycoalcaloids, pH-sensitive field effect transistors, butyrylcholinesterase, thin-layer chromatography.