Biopolymers and cell. 2005; 21 (3): 270 - 274

Development of model DNA-vaccine

I. O. Pokholenko, T. G. Titok, O. M. Sukhorada, T. A. Ruban

The development of DNA-vaccines is one of the most perspective directions in modern vaccionology. However, despite rapid progress in their application, a broad range of questions concerning the basic mechanisms of induction of immune response to DNA-vaccination remains unanswered. A model DNA-vaccine, containing the gene of Eschericha coli b-galactosidase as the gene of model antigen, has been developed. The data obtained suggest that the created recombinant plasmid pBG-TR/02 is able to induce the humoral immune response to E. coli b-galactosidase in mice depending on the place of administration and, therefore, it can be used as the model DNA-vaccine for future research.

Key words: DNA-vaccine, model antigen, immunization, humoral immune response.