Biopolymers and cell. 2005; 21 (3): 264 - 269

Study of exopectate lyase gene of Klebsiella oxytoca VN13 expression

O. V. Lar, G. L. Kovtunovych, N. O. Kozyrovska

The belonging of the pectate lyase pelX of K. oxytoca VN13 to the exopectinase group was shown by paper chromatography. For an analysis of the pelX expression, fusion of the regulatory region of pelX and the coding part of reporter gene lacZ was constructed. The high constitutive level of the pelX expression and its slight inducibility in the presence of plant inducers was determined by the character of the fusion expression in K. oxytoca VN13. The data on regulation of the expression of pectinolytic genes of K. oxytoca VN13 by plant products were obtained.

Key words: Klebsiella oxytoca VN13, exopectate lyase, pelX gene.