Biopolymers and cell. 2005; 21 (2): 151 - 156

The affinity chromatography of glicobiopolymers from sensitive and hypersensitive tobacco plants infected by tobacco mosaic virus

O. G. Kovalenko, A. M. Kyrychenko, T. A. Telegeeva

The extracellular carbohydrate-containing biopolymers which are absent in healthy plants have been discovered in hypersensitive plants by the affinity chromatography method one day after inoculation Such components have not been revealed in tissues of TMV-infected sensitive mutant of the same variety. Since these biopolymers may be specifically bound to concanavalin A, but not to germ wheat agglutinin, they probably contain the mannosa and/or glucosa as carbohydrate components. A possible functional role of these biopolymers in initiation of virus-induced hypersensitive reaction in plants is discussed.

Key words: tobacco mosaic virus, hypersensitive reaction, glicobiopolymers, lectins, affinity chromatography.