Biopolymers and cell. Volume 20. № 6. 524-529.

L. N. Moisa, V. A. Chiljakov

The modeling of the growth curve of the Escherichia coli strain produsing the recombinant b-galactosidase protein

Summary

A kinetics analysis method of growth curves based on the Verhulst logistic function has been used to determine the some growth parameters describing physiological activity of an E.coli strain expressing a recombinant b-galactosidase protein controlled by C1857 gene. The several growth points critical for microbial population development such as transition of increasing growth speed to the decreasing one (the inflection point of the curve – T_e), the maximal growth acceleration phase (the point T₁), the negative growth acceleration (slowing) phase (the point T₂) have been calculated. This approach allows the prediction of the optimal conditions for the cloned gene expression in any age of the culture growth.