Biopolymers and cell. Volume 20. № 4. 300-306.

A. P. Pogribna, S. V. Gorchakov, B. S. Negrutskii

Comparative analysis of the functional activity of the native and modified by trypsin forms of eukaryotic translation elongation factor 1a

Summary

Eukaryotic translation elongation factor 1A (eEF1A) is one of the main components of the translation machinery in cells. It performs also a number of non-canonical functions which are not related to the protein synthesis. In this work eEF1A was subjected to limited trypsinolysis to obtain the truncated by 67 amino acids polypeptide eEF1A similar to a potential product of the oncogene PTI-1. eEF1A was used as a model to study possible translation functions of the PTI-1 oncogene product. Comparison of eEF1A and eEF1A in four different functional tests suggests a possibility of the participation of the PTI-1 protein in translation.