Biopolymers and cell. Volume 20. № 3. 233-238.

L. I. Strokovskaya, R. A. Meleshko, I. M. Kikhno, A. P. Solomko

Purification of intracellular recombinant human prolactin synthesized in insect cells

Summary

Human prolactin (PRL) was expressed in the insect cell culture infected with Autographa californica (Ac) nuclear polyhedrosis virus (NPV) which contained prolactin cDNA under the control of polyhedrin gene promoter. Prolactin was expressed as 6 x His-tagged protein and was purified using Co²⁺-affinity chromatography. The yield of purified protein reached 5 to 50 mg/l depending on the type of insect cells and circumstances of their cultivation. The recombinant prolactin obtained was biologically active.