Biopolymers and cell. 2003; 19 (5): 436 - 439

Trpl44 as a fluorescence probe for investigation of the C-module rapid conformation dynamics in eukaryotic tyrosyle-tRNA synthetase

M. A. Kordysh, K. A. Odynets, A. I. Kornelyuk

The spectra of fluorescence of the tyrosyle-tRNA synthetase C-module have been obtained. The microsurrounding of a tryptophan residue has been characterized using the methods of fluorescence spectroscopy and computational modeling. The rapid conformation dynamics of the C-module in nanosecond time scale is revealed using the fluorescence quenching with acrylamide and Cs ions.