Biopolymers and cell. 2003; 19 (4): 355 - 361

The a2-macroglobulin—trypsin complex as a sensitive element of a potentiometric biosensor for mediated quantitative determination of proteinases

O. A. Beloivan, A. P. Soldatkin

A possibility of application of the a2-macroglobulin (a2M)—trypsin complex as a sensitive element of a potentiometric biosensor for mediated Quantitative determination of proteinases through their esterase activity at the hydrolysis of Na-benzoyl-L-arginin-ethyl ester (BAEE) has been studied. The a2M—trypsin complex immo¬bilization at pH-FET surface in saturated glutaraldehyde (GA) vapours has been optimized. The selective membrane characteristics have been studied. The optimal conditions of measuring BAEE hydrolysis by the biosensor obtained have been determined. A possibility to detect 0.1—1.0 mM BAEE and trypsin activity within the range of 0.1—30 ill ml has been shown.'A conclusion has been made that the membrane developed is promising as a sensitive element of the potentiometric biosensors.