Biopolymers and cell. 2003; 19 (3): 257 - 261

Usage of ISSR and SSR-PCR DNA-marker to Ppd genes

I. A. Balashova, V. I. Feit, Yu. M. Sivolap

SSR- and ISSR-analyses were used for creating a marker to genes responsible for the sensitivity to photoperiod, in particular, the genes PpdDIa and PpdBIa. The gene marker to PpdBIa is a polymorphic SSR-apticon of size 180 b. p. The gene marker to PpdDIa is nulle-allelic 350(-). The genotypes homozygous on PpdDIa are identified by the absence of amplification product of size 350 b. p.