Biopolymers and cell. 2003; 19 (3): 252 - 256

Novel cell line with multiple markers, derivative of CHO-K1

V. A. Kordyum, E. K. Toporova, O. V. Okunev, Y. A. Pokholenka, E. M. Suchorada, T. A. Ruban, V. I. Andrienko, D. M. Irodov

A cell line CHO-K1 /pro-has been consecutively transfected with plasmids pEGFP-Cl and pTR-GT60. A subline with genotype pro^- , neo⁺ , gfp⁺ , tk_hv⁺ , hph⁺ has been obtained after selection in the selective media. The cells are auxotrophic mutants deficient in proline, resistant to antibiotics G-418 and hygromycin B, sensitive to Ganciclovir and produce greenish fluorescence when irradiated with long ultraviolet light.