Biopolymers and cell. 2003; 19 (3): 231 - 237

Isolation and characterization of monoclonal antibodies against eukaryotic translation elongation factor 1A

A. P. Pogrebnaya, N. V. Markeyeva, V. V. Lisogubov, A. M. Fridberg, S. I. Zabashnyi, V. S. Usenko, V. V. Filonenko, P. V. Pogrebnoy, B. S. Negrutskii

Eukaryotic translation elongation factor 1A (eEF1A) is one of the main components of the translation apparatus.The protein also fulfills a number of non-canonical functions not related directly to protein synthesis. In this work the homogenous elongation factor 1A was obtained from hen liver by several chromatographic procedures with different carriers. The purified protein was used to develop monoclonal antibodies. The anti-eEF1A antibodies obtained are species-unspecific, they recognize both eEF1A and eEFIA2 isoforms in ELJSA and immunoblotting. The antibodies have been successfully used for immunohistological studies on human tissues.