Biopolymers and cell. 2002; 18 (6): 529 - 533

The influence of nutrients in growth media on the lysis and extracellular localization of human alpha interferon in the system of recombinant proteins overproduction using bacteriophage lambda

I. Yu. Slavchenko

The influence of different carbon sources on the yield of extra¬cellular human alpha interferon in the system of recombinant proteins biosynthesis using bacteriophage lambda was investigated. Both the level of target protein synthesis and productivity of infected cells lysis are shown to increase in the presence of certain carbon sources in the growth media. This results in rising the extracellular concentration of recombinant protein.