Biopolymers and cell. 2002; 18 (6): 489 - 495

Development of microbiosensors based on carbon fibres for in vivo determination of glucose, acetylcholine and choline

O. M. Schuvailo, L. V. Danyleyko, V. M. Arkhypova, S. V. Dzyadevych, A. V. Elskaya, R. Cespuglio, A. P. Soldatkin

A new improved technology for manufacturing microelectrodes based on carbon fibres has been developed. The microelectrodes produced are long living, stable to electrochemical and chemical treatment. The electrochemically activated microelectrodes demonstrated high sensitivity to H2O2 with the detection limit for hydrogen peroxide of 0.5 fiM. The microbiosensors based on carbon fibre microelectrodes have been developed for the determination of glucose, acetylcholine and choline concentration. They demonstrate high sensitivity in model solutions. The detection limits for glucose, acetylcholine and choline are of 60 uM, 1 uM u 1 uM respectively, that permits these microbiosensors to be applied for in vivo analysis of these substrates in mammalian brain.