Biopolymers and cell. 2002; 18 (5): 406 - 410

Histone hydrolysis in insulated nuclei of carcinoma and in liver cells of rats with Keren's carcinoma at tumor growth

M. M. Marchenko, G. P. Kopitchuk, O. V. Grigorjeva

The features of proteolysis of separate histone fractions in insulated nuclei of carcinoma and in liver cells of rats with Heren's carcinoma were studied at tumor growth as well as upon the usage of modulators of free-radical processes in chromatin. The histone electrophoretic profile of insulated nuclei obtained for the liver cells of rats with Heren's carcinoma did not show qualitative changes In comparison with normal animals. The differences concerned only quantitative redistribution of the linker histone subfractions. The enhanced degradation of the HI histone and H2A core fraction was revealed in the transformed cells nuclei. A phytoreagent activated the destructive processes in the tumor cell chromatin, normalizing the endogenous nuclear proteolysis in liver of the tumor carriers. A chemical reagent 5,6-benzcumarin-S-uracil caused complete disbalance of chromatin molecular organization in the nuclei of the carcinoma and liver cells during the oncogenesis.