Biopolymers and cell. 2002; 18 (4): 340 - 346

 

 

Interaction of cyanine dyes with nucleic acids. New (pyrido)(thio)trimethincyanine dye CCyan 40 for fluorescent labeling of oligonucleotides

 

S. M. Yarmoluk, M. Yu. Losytskyy, V. B. Kovatska, S. M. Tomachynski, T. Yu. Ogutchansky, 0. M. Kostenko, V. V. Kurdyukov, O. I. Tolmachev

 

The spectral-luminescent characteristics of the CCyan 40 carbo-cyanine dye in free state and in the presence of DNA and synthetic polynucleotides werp studied. The dye has high molar extinction coefficient (5.7-JO M cm ) and average quantum yield value (0.042). In the presence of DNA, poly(dGCldGC) and po-ly(dAldT) the quantum yield of CCyan 40 increases in 5.5, 3.8 and 1.8 times respectively. We recommend the dye CCyan 40 for the fluorescent labeling of oligonucleotides using the reaction, proposed by us earlier.