Biopolymers and cell. 2002; 18 (4): 334 - 339

The influence of organic carbon sources on the production of a recombinant protein in Escherichia coll cells

I. Yu. Slavchenko

The influence of different carbon sources in growth media on the production of a recombinant protein (IFN) in E. coll cells was investigated. It was ascertained that upon the cultivation of the IFN producer E. coll SG30 (pIF-16) at 37 °C the carbon sources content in the media may cause either increase or decrease in the yield of target product. The level of recombinant protein production in¬creases when glycerol, rhamnose, fructose, sorbitol are added to the cultivation media, while glucose, maltose, mannitol, xylose de¬creases it. The negative influence of these carbon sources was shown to be temperature-dependent and followed by strong reduction in pH of medium. Possible mechanisms of influence of carbon sources in the media on the production of a recombinant protein in E. call cells are discussed.