Biopolymers and cell. 2002; 18 (2): 135 - 138

Development of the plate assay screening procedure for isolation of the mutants deficient in inactivation of peroxisomal enzymes in the yeast Yarrowia lipolytica

T. Y. Nazarko, M. J. Mala, A. A. Sibirny

The amine oxidase (AMO) plate assay screening procedure for isolation of the mutants deficient in inactivation of peroxisomal enzymes in the yeast Y. lipolytica has been developed. The first tagged mutants affected in the peroxisomal AMO and isocitrate lyase inactivation were generated by the insertion of a zeta-URA3 mutagenesis cassette into the genome of a zeta-free and ura3 deletion mutant strain of Y. lipolytica.