Biopolymers and cell. 2002; 18 (2): 96 - 101

Mutation analysis and bacterial expression of the chimerical oncoprotein Bcr/Abl Dbl-homology domain

A. N. Dubrovskaya, G. D. Telegeev, M. V. Dybkov, O. S. Voloshanenko, V. V. Shved, S. S. Maliuta

We obtained the differences of the acttn distribution in the cell from health donors (diffuse cytoplasmic distribution), patients with ALL, PhB-positive human cell line K362 and most cases CML blast crisis (cortical F-actin distribution) and in the one case of CML blast crisis a dot- like actin structures were observed. The revealing of dot-like actin distribution correlated with detection of mutations in dbl part of bcr/abl gene. Recombinant Dbl domain of Bcr/Abl was expressed in pET32b expression system and used for obtaining of the polyclonal antibody. Obtained data may cast light on the nature of CML blast crisis development and can be used for early detection of CML tumor progression.