Biopolymers and cell. 2002; 18 (1): 62 - 70

Investigation of aminomodified tips for atomic force microscopy of biomolecules

A. P. Limanskii

Atomic force microscopy (AFM) was used for characterization of aminomodified tips in buffer solutions with different ionic strength (I) and pH values. Optimal conditions for tip modification in aminosilane vapour for following DNA immobilization were determined. Adhesion forces have been decreased for pair aminomodified tip — mica from I - 10 mM to I - 100 mM and pH 11.2. Functionalization of aminomodified tips by binding with glutaraldehyde and interaction with DNA was shown.