Biopolymers and cell. 2001; 17 (5): 401 - 405

Physicochemical properties of the polyhedrin of Selenephera lunigera nuclear polyhedrosis virus

E. A. Koslov, T. L. Levitina, M. T. Bobrovskaya, M. N. Ovander

The polyhedrin was obtained by polyhedra dissolution according to the alkaline (pH 10.5) and acetic acid (67 % CH^COOH) methods. It was shown by polyacrilamid gel electrophoresis that acetic polyhedrin contained one component of molecular weight (m. w.) 25000 while alkaline polyhedrin contained 28500-component and its fragments of m. w. 8000, 10000, 14000, 19000, 25000. It was shown by ultracentrifugation that acetic acid polyhedrin revealed in 67 % CH3COOH one monomer component with sedimentation coefficient of 1,9S and alkaline polyhedrin revealed in pHW.5 solution three oligomeric components with sedimentation coefficients of 13S (70 %), 19S (20 %) and 23S (JO %). It was shown by the Dansyl method that alkaline protein contained six N-terminal amino acid residues: Met, Val, Leu, Thr, Lys while Acetic acid polyhedrin had no N-terminal NH2-group. The peptide map of alkaline polyhedrin was prepared, and amino acid composition of acetic acid polyhedrin was determined.