Biopolymers and cell. 2001; 17 (1): 80 - 84

Peptides and cyanine dyes. 2. Covalent labelling of amino acids and peptides by means of the reaction of pyrylium cyanine dyes with amines

O. M. Kostenko, S. U. Dmitrieva, S. M. Yarmoluk

Here we propose a new amino-reactive pyrtlium dye for the conjugation with peptides through N-terminal and N-e-lysine's aminogroups. The reactivity of Cyan 39 towards eleven most interesting aminoacids has been investigated in triethylammonium acetate and sodium carbonate-bicarbonate buffers. The conjugation with 72-aminoacid model peptide has been carried out successfully.