Biopolymers and cell. 2000; 16 (6): 505 - 509

Catalytic properties of the Penicilium vitate catalase. Peroxidatic reaction of the enzyme

N. V. Latyshko, L. V. Gudkova, O. A. Gudkova

Peroxidatic activity of the P. vitale catalase with ethanol as a substrate was investigated. It was determined that the peroxidatic activity of the catalase (5—13 U/mg) was over a hundred times less the catalatic activity. Study of the steady-state kinetics showed that kinetic parameters of peroxidatic reaction -Km 5.9 mM and Vmix 0.22 mM/min were lower tlian estimated earlier kinetics of Jlitj catalytic reaction. However, the turnover number _AjclU -1.1-10 s and catalatic efficiency kM/Km ratio 1.9-10 M s testify the catalase is rather effective peroxidase. The enzyme /fas the rate constant of the peroxidatic action equal to 2.4-10 s M as catalases of other origins.