Biopolymers and cell. 2000; 16 (4): 320 - 326

The interaction of cyanine dyes with nucleic acids. 16. New fluorescent benzothiazole-4-(2,6-dimethylpyridinium] monomethyne cyanine dyes

D. V. Kryvorotenko, V. B. Kovalska, S. M. Yarmoluk

New fluorescent probes for nucleic acids detection based on 2-[l-(5-carboxypentyl)-2,6-dimethyl-J,4-dihydro-4-pyridinylidenmethyl]-3-methyl-J,3-benzothiazole-3-ium perchlorate have been synthesized. The spectral properties of the compounds obtained and their complexes with nucleic acids have been investigated. The influence of different functional substituents in the dye molecule on its interaction with nucleic acids has been studied. The results obtained show that the inclusion of hydroxy- groups and acridine fragment in the structure of cyanine increases the sensibility of the nucleic acids detection.