Biopolymers and cell. 2000; 16 (3): 229 - 235

Bacterial expression of full-length and truncated forms of cytokine EMAP-2 and cytokine-like domain of mammalian tyrosyl-tRNA synthetase

A. L. Dubrovsky, Jn, Brown, A. I. Kornelyuk, J. C. Murray, G. Kh. Matsuka

DNA fragments encoded full-length cytokine EMAP-2 and cytokine-like domain of mammalian TyrRS and truncated from COOH- and NH2-termini forms were cloned in vector for bacterial expression pET32a. For all clones bacterial overexpression were obtained and full-length forms were purified to homogeneity state by affin chromatography and affinity tag was remove by protease cleavage. Induction of tissue factor for full-length forms was tested. Full-length form of cytokine-like domain of mammalian TyrRS did not interact with polyclonal antibody against EMAP-2 in western-blot analysis. It was concluded that this set of recombinant proteins may be usefull for functional study of these cytokine proteins.