Biopolymers and cell. 2000; 16 (2): 115 - 123

Study of tertiary structure elements of tRNA from Scr Thermits thermophilus in solution

O. P. Kovalenko, I. A. Kriklivyi, M. A. Tukalo

Reactivity of phosphates and bases in tRNA T. thermophilus has been studied by chemical modification methods. Ethylnitrosourea has been used to modify phosphate groups, guanosines and cytidines have been modified by dimethylsulphate, adenosines — by diethylpy-rocarbonate. The same tertiary interactions exist in the solution structure of tRNA T. thermophilus as in the crystal one.