Biopolymers and cell. 1999; 15 (6): 538 - 542

Analysis of protein-protein interactions mediated by adaptor protein Grb2 during the course of megakaryocytic differentiation of human erythroleukemia cell line K562

O. M. Il'nytska, O. Yu. Palyvoda, Z. M. Gusak, T. I. Val'ovka, N. I. Igunwntseva, S. 1. Kusen', L. B. Drobot

Protein-protein interactions mediated by adaptor protein Grb2 during the course of PMA-induced megakaryocytic differentiation of the human erythroleukemia cell line H562 were studied. Binding assay was performed in vitro using the recombinant forms of Grb2, (Crb2/full, Grb2/N-SH3 and Grb2/C-SH3), conjugated with gluta-thione-S-transferase. Proteins, binding of which are modulated in differentiated cells, were identified and binding dynamics was analysed. It is supposed that a set of Grb2 targets could be used in common in signalling pathways involved in both the stimulation of mitogenesis and the induction of differentiation in the K562 cells.